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Image Search Results
Journal: bioRxiv
Article Title: ZNF768 regulates expression of E2F1 protein to drive G0/G1 transition and cell cycle progression
doi: 10.64898/2026.08.03.742597
Figure Lengend Snippet: A Western blots were performed on mitotic lysates generated from the panel of cell lines depleted of ZNF768 with inducible sh_ZNF768_1 ( n =3). B Representative immunofluorescence image of mitotic inducible HeLa cells after 48 hours doxycycline induction. Cells were stained for PLK1 (green), CENPC (grey) and with Hoechst (blue). C Quantification of the average relative intensity of PLK1 at kinetochores from ( B ) using previous inducible cancer cell lines ( n =3). D Representative stills of time-lapse movies of inducible HeLa cells after a 48 hours ZNF768 knockdown. For imaging, cells were synchronized in mitosis after release from a double thymidine block and incubated with SiR-DNA to visualize the chromatin. E Quantification of the average nuclear envelope breakdown to the anaphase onset duration in the indicated cell lines ( n = n=3 for all cell lines except for the HeLa, n=4). F Representative live cell images of mitotic defects observed after 48 hour ZNF768 depletion in HeLa cells. G Quantification of mitotic defects listed in F in MDA-MB-231, HeLa, HCT-116 and U87 cell lines after 48 hour ZNF768 depletion ( n =3). In all panels, data represent the mean ± SEM. In panel C , E and G , significance was determined by unpaired t test.
Article Snippet: For live-cell imaging, cells were washed with PBS and treated with
Techniques: Western Blot, Generated, Immunofluorescence, Staining, Knockdown, Imaging, Blocking Assay, Incubation